Discussion The present study indicated that in hyperthyroid

2.9. Statistical analysis
CK and LDH values are expressed as meansuuSEM. Statistical analysis was performed using SPSS 16 software. One-way ANOVA with Tukey\'s HSD post hoc test was used for comparing differences of CK and LDH means between different groups. MannaWhitney U test was used to compare gene expression between two groups.
3. Results
3.1. Characteristics of the animals
The CK-MB and LDH levels in the coronary effluent of both control and hyperthyroid animals are shown in Fig.1. In both groups, IR significantly (pu<u0.05) increased CK-MB and LDH levels in coronary flow; following IR, hyperthyroid rats had significantly higher levels of CK-MB and LDH than controls (pu<u0.05). IPost partially restored elevated levels of both BMS-303141 in the C-IR, but not in the HP-IR group. In the control group, IPost in the presence of AG had no significant additive effect in reducing CK-MB and LDH levels. In addition, IPost used in combination with AG restored elevated enzymes in hyperthyroid rats.
Fig.1.uCK-MB (A) and LDH (B) levels in coronary flow in first 10umin after reperfusion. C, control; IR, ischemiaareperfusion; IPost, ischemic postconditioning; HP, hyperthyroidism; and AG, aminoguanidine; data are meanuuSE (nu=u8 rats in each group); *pu<u0.05 compared with control group. ‡pu<u0.05 compared with C-IR group. #pu<u0.05 compared with hyperthyroid group. †pu<u0.05 compared with HP-IPost group.Figure optionsDownload full-size imageDownload high-quality image (902 K)Download as PowerPoint slide
Fig.2.uElectrophoretic pattern of DNA laddering in heart tissue. Marker 100ubp; C, control; IR, ischemiaareperfusion; IPost, ischemic postconditioning; HP, hyperthyroidism; image and AG, aminoguanidine.Figure optionsDownload full-size imageDownload high-quality image (270 K)Download as PowerPoint slide
Fig.3.uThe changes of infarct size in hearts of control and hyperthyroid groups. C, control; IR, ischemiaareperfusion; IPost, ischemic postconditioning; HP, hyperthyroidism; and AG, aminoguanidine; data are meanuuSE; (nu=u8 rats) as present of total area; *pu<u0.05 compared with control group. ‡pu<u0.05 compared with C-IR group. #pu<u0.05 compared with hyperthyroid group. †pu<u0.05 compared with HP-IPost group.Figure optionsDownload full-size imageDownload high-quality image (518 K)Download as PowerPoint slide
As shown in Fig.2, following IR, a DNA laddering pattern, a biochemical characteristic of apoptosis indicating the cleavage of chromatin into little fragments, was observed in both the control and hyperthyroid groups. IPost decreased IR-induced DNA laddering in controls but not in the hyperthyroid group. In addition, IPost reduced IR-induced DNA laddering in hyperthyroid rats when used in combination with AG.
3.5. Effects of IPost on gene expression
Heart mRNA levels of both eNOS and iNOS were significantly (pu<u0.05) higher in hyperthyroid rats, compared to controls (1.73 and 2.09 fold respectively) while those for Bax and Bcl-2 were comparable. Following IR, expression of eNOS and Bcl-2 were decreased while expression of iNOS and Bax were increased in both groups. IPost increased the expression of eNOS and Bcl-2 by 3.24 and 3.61 fold and decreased expression of iNOS and Bax by 0.62 and 0.21 fold in controls only, but not in the hyperthyroid rats. In hyperthyroid rats, effects of IPost, in the presence of AG on gene expression were similar to those observed in controls (Table2).
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